Production and characterization of a hybridoma-derived antibody to Blastomyces dermatitidis.
نویسندگان
چکیده
A hybridoma cell line was isolated which produced monoclonal antibody to one protein component of a yeast-phase cytoplasmic antigenic complex of Blastomyces dermatitidis. The immunoglobulin M antibody product was characterized by immunodiffusion, autoradiography of polyacrylamide gels, and cellulose acetate electrophoresis. By attaching the antibody to an affinity gel, one major protein band was identified by polyacrylamide gel electrophoresis as the antigen for which the antibody was specific.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 15 2 شماره
صفحات -
تاریخ انتشار 1982